Contact an Account ManagerMeasurement of urinary catecholamines, their catechol metabolites and precursors by liquid chromatography with column switching and on-line fluorimetric and electrochemical detection
Summary
Type: HPLC
Application #: 101850
Catalog#: 720140.46 - MN HPLC Column, Nucleosil C18, 100A, 10um, 4.6x150mm
Detection: FD = fluorescence, excitation 282nm, emission 314nm,
ED = electrochemical, glassy carbon electrode at +0.72V versus Ag/AgCl
Conditions: eluent: 0.06M KH2PO4, 50mg/l disodium ethylenediaminetetraacetate, 100mg/l octyl sulphate used as ion pairing agent and 12.5% methanol, adjusted to pH 3.5 at 22°C with 5M phosphoric acid,
flow rate 0
Authors: Julien, C.
Source: J. Chromatogr. 344 (1985) 51–60
Details
Legend: (a) standard solution containing:
60ng/ml DOPA,
150ng/ml NA,
45ng/ml A,
750ng/ml DA,
1000ng/ml isoproterenol (int. std.),
(b) of a human urinary extract after adsorption onto alumina.